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If inconsistencies occur, look for the following sources of error: Several other publications Metabolism of a mouse useful information on similar issues that are directly pertinent to the measurement of energy metabolism in the mouse and the reader may also wish to consult these, in particular the papers by WeirKleiberFerranniniSimonson and deFronzoBursztein et al.
Practice this technique until the measured time interval appears consistent. Two types of system are available — one of which involves a single small Spartan chamber linked to a single analyzer, which is ideal for measuring the individual components of energy demand.
Accurately weigh the wire cage, place a mouse in the cage, and reweigh both. This includes characterization of the factors that influence energy expenditure and dysregulation of energy balance leading to obesity and its sequelae. Ability to accurately measure the total daily expenditure or the different components depends on the type of equipment available.
This may not be the case. The rat is also a mammalian endotherm that shares much of its genome and physiology with the human. Seal the chamber by applying a drop of bubble solution to the end of the pipette. Blood samples will be collected at 0, 0.
In this experiment we will test one of two possible hypotheses: This paper considers some theoretical, practical, and analytical considerations to be considered when measuring energy expenditure in mice.
The greatest analytical challenge for mouse expenditure data is how to account for body size differences between individuals. However, a fundamental assumption being made in this type of diagram is that the components, as defined, are independent and additive.
It also addresses the issue of how to analyze the resulting data and some of the pitfalls in this analysis. At min following the injection, the mouse will be anesthetized, and samples will be collected. The use of these instruments allows longitudinal measurements of key metabolic parameters in the same cohort of animals at different ages and under different feeding conditions, and will enable the accumulation of data over several months of time.
These latter systems are ideal for measuring total daily energy expenditure but at present do not allow accurate decomposition of the total expenditure into its components.
Cover the bottom of the metabolism chamber with a thin layer of soda lime so that all exhaled carbon dioxide will be absorbed, differentially allowing for the measurement of oxygen consumption, only.
Repeat the procedure for a total of 3 trial runs. Imagine a 30 g mouse is in a direct calorimeter and it is expending 0.
Research laboratories are provided with ready accessand training in the proper use of the equipment. Organ-specific triglyceride levels will be measured using spectrophometry, and [14C]-labeled triglyceride will be measured using liquid scintillation Protein metabolism in individual organs in conscious mice In vivo rates of protein synthesis in individual organs heart, skeletal muscle, liver, adipose tissue, lung, and kidney will be measured in awake mice by determining the Metabolism of a mouse of L-[3H]Phenylalanine incorporation into proteins.
Basal whole body and organ-specific glucose metabolism will be calculated as described in the Hyperinsulinemic-euglycemic clamp study. Use a stopwatch to determine the time in seconds required for the soap bubbles to traverse a distance along the pipette equivalent to exactly 5 ml. Allow the animal to remain in the chamber for about 10 minutes for temperature equilibration.
A spacing device, such as a cardboard tube, placed in the cage will confine the animal, minimizing physical activity. In order to determine the metabolic rates for each animal, you will need to calculate the metabolic rate using the oxygen consumption rate you measure in class.
This procedure will later reduce the possibility of the soap-bubble seal drying out and breaking during migration. This is called direct calorimetry. Wet the rubber stopper to insure a tight seal on the metabolism chamber. The error induced by this assumption can be quite large.Dec 28, · We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof.
The mission of the UCSF Nutrition & Obesity Research Center is to provide a cohesive and efficient support of basic, translational and clinical research programs focused on nutrition, obesity or metabolism at UCSF. To identify the function of genes, the IMPC uses a series of standardised protocols described in IMPReSS (International Mouse Phenotyping Resource of Standardised Screens).
Tests addressing the metabolic function are conducted on young adult mice at weeks of age. I´m trying to orientate in the problematics of cholesterol metabolism. I´ve heard there are crucial differences between cholesterol metabolism in mouse and human, but I. Metabolism Core Measurement of food intake, energy expenditure and activity using metabolic cages Metabolic cages (TSE Systems; photo) will be used in awake mice to simultaneously measure energy expenditure, physical activity, indirect calorimetry, and food/water intake.
Mar 14, · The mouse is one of the most important model organisms for understanding human genetic function and disease. This includes characterization of the factors that influence energy expenditure and dysregulation of energy balance leading to obesity and its sequelae.
Measuring energy metabolism in the.Download